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KMID : 0545119920020040278
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Journal of Microbiology and Biotechnology
1992 Volume.2 No. 4 p.278 ~ p.283
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Biocatalytic Production of Aldehyde by a Methanol Utilizing Yeast, Hansenula nonfermentans KYP-1 Grown in Methanol-limited Continuous Culture
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Yoon, Byung Dae
Kim, Hee Sik/Kwon, Tae Jong/Yang, Ji Won/Kwon, Gi Seok/Lee, Hyun Sun/Ahn, Jong Seog
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Abstract
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Aldehyde production by cells of a methanol utilizing yeast, Hansenula nonfermentans KYP-1 was improved when they were grown in a methanol-limited continuous culture, in comparison with cells grown in a batch culture. A higher cell yield was also obtained in continuous culture than in batch culture. This could be due to the fact that a lower methanol concentration was maintained in the jar fermentor to minimize growth inhibition by methanol. A maximum cell productivity of 0.219 g·liter^-1·hr^-1 and a cell yield of 47% were obtained at dilution rates of 0.1 hr^-1 and 0.06 hr^-1, respectively. The greatest amount of aldehyde was measured at a dilution rate of 0.08 hr^-1. Under optimum reaction conditions, 915.7 mM of acetaldehyde was produced from 1.5 M ethanol after 21 hours´ reaction, with a conversion rate of 61%. Propionaldehyde and acrolein were produced with conversion rates of 32.7% and 44%, respectively.
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